Composite

Part:BBa_K4131012:Design

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-10)


The lac-induced expressing cassette of AccBC, AccD1 and AccE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3976
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1568
    Illegal XhoI site found at 317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 925
    Illegal NgoMIV site found at 1299
    Illegal NgoMIV site found at 1789
    Illegal AgeI site found at 663
    Illegal AgeI site found at 1165
    Illegal AgeI site found at 1948
    Illegal AgeI site found at 2330
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The AccBC (BBa_K4131001), AccD1 (BBa_K4131000) and AccE (BBa_K4131002) genes are codon optimized for biobrick assembly


Source

The lac promoter and operon (Bba_R0010) are located at the 5' terminal of this composite part to regulate the AccBC, AccD1 and AccE genes transcription sequently. The t7 terminator (Bba_K3778018) is t the 3' terminal of this composite part.

The ribosome binding site (Bba_K3778013) is respectively added before AccBC, AccD1 and AccE genes to mediated the individual protein translation.

References